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glut3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc glut3
    Glut3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glut3/Glut3+Rabbit+mAb/10__1016_slash_j__jri__2026__104973-64-22-24
    Average 94 stars, based on 13 article reviews
    glut3 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Polyacrylamide Gel Electrophoresis:

    Article Title: Selinexor targets expression of metabolic genes in Merkel cell carcinoma cells.
    Article Snippet: .. MS-1 cell lines (Sigma-Aldrich, #9,111,802) were treated with increasing doses of 25, 100, 500, and 750 nM of selinexor for 72 h. Sodium dodecyl sulfate polyacrylamide gel electrophoresis system (SDS-PAGE) and Western blotting were performed prior to detection by antibodies against aldolase A (#8060, Cell Signaling Technology (CST), Danvers, MA, USA), amphiregulin (sc-74,501, Santa Cruz Biotechnology (SCB), Dallas, TX, USA), enolase 1 (#3810, CST), G6PD (#12,263, CST), GLUD1 (ARC1216, ThermoFisher Scientific, Waltham, MA, USA), GLUT3 (sc-74,497, SCB), Glutaminase (#49,363, CST), Hexokinase 1 (#2024, CST), LDHA (#3582, CST), MCT1 (#76,508, CST), PDHK1 (#3820, CST), PFKFB2 (#13,045, CST), PFKFB3 (#13,123, CST), 6PGD (#2263, CST), and PKM2 (#4053, CST). .. The antibody glyceraldehyde 3-phosphate (GAPDH) (GT239, GeneTex, Irvine, CA, USA) was used as the loading control.

    Article Title: Selinexor targets expression of metabolic genes in Merkel cell carcinoma cells
    Article Snippet: Materials and Methods MS-1 cell lines (Sigma-Aldrich, #9111802) were treated with increasing doses of 25, 100, 500, and 750 nM of selinexor for 72 hours. .. Sodium dodecyl sulfate polyacrylamide gel electrophoresis system (SDSPAGE) and Western blotting were performed prior to detection by antibodies against aldolase A (#8060, Cell Signaling Technology (CST), Danvers, MA, USA), amphiregulin (sc-74501, Santa Cruz Biotechnology (SCB), Dallas, TX, USA), enolase 1 (#3810, CST), G6PD (#12263, CST), GLUD1 (ARC1216, ThermoFisher Scienti c, Waltham, MA, USA), GLUT3 (sc-74497, SCB), Glutaminase (#49363, CST), Hexokinase 1 (#2024, CST), LDHA (#3582, CST), MCT1 (#76508, CST), PDHK1 (#3820, CST), PFKFB2 (#13045, CST), PFKFB3 (#13123, CST), 6PGD (#2263, CST), and PKM2 (#4053, CST). .. The antibody glyceraldehyde 3- phosphate (GAPDH) (GT239, GeneTex, Irvine, CA, USA) was used as the loading control.

    Western Blot:

    Article Title: Selinexor targets expression of metabolic genes in Merkel cell carcinoma cells.
    Article Snippet: .. MS-1 cell lines (Sigma-Aldrich, #9,111,802) were treated with increasing doses of 25, 100, 500, and 750 nM of selinexor for 72 h. Sodium dodecyl sulfate polyacrylamide gel electrophoresis system (SDS-PAGE) and Western blotting were performed prior to detection by antibodies against aldolase A (#8060, Cell Signaling Technology (CST), Danvers, MA, USA), amphiregulin (sc-74,501, Santa Cruz Biotechnology (SCB), Dallas, TX, USA), enolase 1 (#3810, CST), G6PD (#12,263, CST), GLUD1 (ARC1216, ThermoFisher Scientific, Waltham, MA, USA), GLUT3 (sc-74,497, SCB), Glutaminase (#49,363, CST), Hexokinase 1 (#2024, CST), LDHA (#3582, CST), MCT1 (#76,508, CST), PDHK1 (#3820, CST), PFKFB2 (#13,045, CST), PFKFB3 (#13,123, CST), 6PGD (#2263, CST), and PKM2 (#4053, CST). .. The antibody glyceraldehyde 3-phosphate (GAPDH) (GT239, GeneTex, Irvine, CA, USA) was used as the loading control.

    Article Title: Selinexor targets expression of metabolic genes in Merkel cell carcinoma cells
    Article Snippet: Materials and Methods MS-1 cell lines (Sigma-Aldrich, #9111802) were treated with increasing doses of 25, 100, 500, and 750 nM of selinexor for 72 hours. .. Sodium dodecyl sulfate polyacrylamide gel electrophoresis system (SDSPAGE) and Western blotting were performed prior to detection by antibodies against aldolase A (#8060, Cell Signaling Technology (CST), Danvers, MA, USA), amphiregulin (sc-74501, Santa Cruz Biotechnology (SCB), Dallas, TX, USA), enolase 1 (#3810, CST), G6PD (#12263, CST), GLUD1 (ARC1216, ThermoFisher Scienti c, Waltham, MA, USA), GLUT3 (sc-74497, SCB), Glutaminase (#49363, CST), Hexokinase 1 (#2024, CST), LDHA (#3582, CST), MCT1 (#76508, CST), PDHK1 (#3820, CST), PFKFB2 (#13045, CST), PFKFB3 (#13123, CST), 6PGD (#2263, CST), and PKM2 (#4053, CST). .. The antibody glyceraldehyde 3- phosphate (GAPDH) (GT239, GeneTex, Irvine, CA, USA) was used as the loading control.

    Control:

    Article Title: Elevated Glucose Levels Trigger Trophoblast Cell Invasion Dysfunction through Enhanced Senescence and IGFBP4 Signaling Pathway
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Immunohistochemistry:

    Article Title: Impact of vitamin A on aged people's cognition and Alzheimer's disease progression in an animal model.
    Article Snippet: IHC assays were performed on brain paraffin-embedded sections using antibodies directed against GLUT1 (1:100 dilution, cell signaling technology, USA), GLUT3 (1:200 dilution, cell signaling technology, USA), and GFAP (1:1200, Servicebio, China). .. IHC assays were performed on brain paraffin-embedded sections using antibodies directed against GLUT1 (1:100 dilution, cell signaling technology, USA), GLUT3 (1:200 dilution, cell signaling technology, USA), and GFAP (1:1200, Servicebio, China). .. Then, the section was observed and photographed by an electric microscope (Olympus BX61, Japan).

    Article Title: Effects of distinct n-6 to n-3 polyunsaturated fatty acid ratios on insulin resistant and AD-like phenotypes in high-fat diets-fed APP/PS1 mice.
    Article Snippet: Type 2 diabetes mellitus (T2DM) and Alzheimer’s disease (AD) are prevalent diseases with similar pathophysiological characteristics and genetic predispositions.. Polyunsaturated fatty acids (PUFAs) are essential in maintaining normal brain function.. However, little is known about the effect of dietary n-6/n-3 PUFA ratio on AD-like pathology, particularly in high-fat diet (HFD)-fed AD model mice.

    Article Title: Impact of vitamin A on aged people’s cognition and Alzheimer’s disease progression in an animal model
    Article Snippet: .. IHC assays were performed on brain paraffin-embedded sections using antibodies directed against GLUT1 (1:100 dilution, cell signaling technology, USA), GLUT3 (1:200 dilution, cell signaling technology, USA), and GFAP (1:1200, Servicebio, China). ..

    Incubation:

    Article Title: Feto-placental endothelial dysfunction in Gestational Diabetes Mellitus under dietary or insulin therapy
    Article Snippet: .. After washing with PBS, enough drops of primary antibody for GLUT1 (Servicebio, rabbit polyclonal antibody), GLUT3 (Cellsignal, rabbit monoclonal antibody), p-AMPKα (Abcam, rabbit monoclonal antibody), p-AKT (Cellsignal, rabbit monoclonal antibody), and cleaved caspase-3 (Cellsignal, rabbit monoclonaln antibody) were separately added and incubated for 1 h. The secondary antibody was added thereafter. ..



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    Proteintech slc2a3
    Correlation between the five signature genes . ( A ) Chord diagram of correlation. ( B ) Heat map of correlation. ( D–M ) Scatter plot of correlation among AKR1C2, SLC2A14, FTL, TNFRSF12A, and <t>SLC2A3.</t> * represents p<0.05; **represents p<0.01; ***represents p<0.01.
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    Image Search Results


    Correlation between the five signature genes . ( A ) Chord diagram of correlation. ( B ) Heat map of correlation. ( D–M ) Scatter plot of correlation among AKR1C2, SLC2A14, FTL, TNFRSF12A, and SLC2A3. * represents p<0.05; **represents p<0.01; ***represents p<0.01.

    Journal: International Journal of General Medicine

    Article Title: A Five-Gene PANoptosis Signature Correlates with Immune Infiltration and Secondary Brain Injury in Intracerebral Hemorrhage

    doi: 10.2147/IJGM.S581629

    Figure Lengend Snippet: Correlation between the five signature genes . ( A ) Chord diagram of correlation. ( B ) Heat map of correlation. ( D–M ) Scatter plot of correlation among AKR1C2, SLC2A14, FTL, TNFRSF12A, and SLC2A3. * represents p<0.05; **represents p<0.01; ***represents p<0.01.

    Article Snippet: After blocking with 5% BSA, the membranes were incubated overnight at 4°C with the following primary antibodies (all at 1:1000 dilution): AKR1C2 (#13035S, Cell Signaling Technology), FTL (#ab69090, Abcam), SLC2A14 (#PK35494-S, Abmart), SLC2A3 (#20403-1-AP, Proteintech), TNFRSF12A (# AWA10766 , Abiowell), and GAPDH (#ab181602, ProteinTech).

    Techniques:

    Experimental verification of protein expression in the clinical sample. ( A ) The expression levels of AKR1C2, SLC2A14, FTL, TNFRSF12A, and SLC2A3 were detected by Western blot. ( B–F ) Protein quantification of the five signature genes. * represents p<0.05; ns represents no significance.

    Journal: International Journal of General Medicine

    Article Title: A Five-Gene PANoptosis Signature Correlates with Immune Infiltration and Secondary Brain Injury in Intracerebral Hemorrhage

    doi: 10.2147/IJGM.S581629

    Figure Lengend Snippet: Experimental verification of protein expression in the clinical sample. ( A ) The expression levels of AKR1C2, SLC2A14, FTL, TNFRSF12A, and SLC2A3 were detected by Western blot. ( B–F ) Protein quantification of the five signature genes. * represents p<0.05; ns represents no significance.

    Article Snippet: After blocking with 5% BSA, the membranes were incubated overnight at 4°C with the following primary antibodies (all at 1:1000 dilution): AKR1C2 (#13035S, Cell Signaling Technology), FTL (#ab69090, Abcam), SLC2A14 (#PK35494-S, Abmart), SLC2A3 (#20403-1-AP, Proteintech), TNFRSF12A (# AWA10766 , Abiowell), and GAPDH (#ab181602, ProteinTech).

    Techniques: Expressing, Western Blot